NAPLEX (North American Pharmacist Licensure Examination) Practice Exam

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Which of the following is associated with 'Atypicals' that do not gram stain well?

  1. Corynebacterium spp.

  2. E. coli

  3. Chlamydia spp.

  4. C. difficile

The correct answer is: Chlamydia spp.

"Atypicals" that do not gram stain well are typically microorganisms that possess cell walls that do not readily retain the crystal violet stain used in the Gram staining technique. Among the options provided, Chlamydia spp. is associated with atypicals that do not gram stain well. Chlamydia is an example of a bacterium that has a unique cell wall composition, causing it to not retain the Gram stain effectively. This characteristic is important to recognize when diagnosing and treating infections caused by Chlamydia. The other options listed (Corynebacterium spp., E. coli, and C. difficile) are not known for being atypicals that do not gram stain well. Corynebacterium spp., E. coli, and C. difficile are typically more easily detectable by Gram staining due to their cell wall compositions, unlike Chlamydia spp.